The invention belongs to the field of hygiene products, and aims to provide a safe and efficient antibacterial hand sanitizer product processed by modern biotechnology. The hand sanitizer provided by the invention comprises a surface active agent, glycerol, an auxiliary agent and deionized water; and the hand sanitizer further comprises 0.1-1 wt% of a bactericide of an extract from Bacillus subtilis fermentation broth. The hand sanitizer provided by the invention realizes dirt removing ability, good sterilization and disinfection function, and high safety. The bactericide added in the invention has antiseptic function, and is safe and efficient; therefore, the hand sanitizer does not the need to be added with preservatives.